aseptic technique Flashcards | Quizlet
Identify the best definition of aseptic technique. A basic procedure used to prevent contamination of the inoculation tools and the working environment basic culture technique streak plate Grinding To prevent contamination of the culture with environmental microbes basic culture technique streak plate Grinding What is the advantage of using the streak plate method over the pour plate method? It is very easy to do.What is the Difference Between Streak Plate and Pour Plate basic culture technique streak plate GrindingJul 21, 2019 · Streak plate is a technique in microbiology used to isolate colonies from a culture. In this technique, a loopful from the bacterial slant is systematically streaked on a nutrient broth. Therefore, it produces surface colonies. Also, this method is important to isolate pure species from a mixture.Streaking culture plates in bacteriologyMay 07, 2016 · Streak plates 1.With this method, a small amount of sample is placed on the side of the agar plate (either with a swab, or as a drop from an inoculating loop if the sample is a liquid). 2.A sterile loop (flamed until red hot, then cooled by touching the agar away from the inoculated sample) is then used to spread the bacteria out in one direction from the initial site of inoculation.This is done by
Streaking Technique Obtain Pure Cultures | Cultivation of basic culture technique streak plate Grinding
Roll the swab over a small 1½-cm square of surface of a blood agar plate, near but not touching one edge (see fig. 9.1, area A). Rotate the swab fully in this area. Discard the swab in a container of disinfectant. Using an inoculating loop, streak the plate as in figure 9.1. Incubate the plate Streak plate procedure1. Flame the loop and wire and streak a loopful of broth as at A in the diagram. 2. Reflame the loop and cool it. 3. Streak as at B to spread the original inoculum over more of the agar. 4. Reflame the loop and cool it. 5. Streak as at C. 6. Reflame the loop and cool it. 7. Streak as at D. 8. Label the plate and incubate it inverted.Streak Plate Method: Principle, Procedure, Uses Microbe basic culture technique streak plate GrindingJul 16, 2016 · Immediately streak the inoculating loop very gently over a quarter of the plate using a back and forth motion (see area 1 in the figure above). Flame the loop again and allow it to cool. Going back to the edge of area 1 that you just streaked, extend the streaks into the second quarter of the plate Reviews: 29Estimated Reading Time: 3 mins
Streak Plate Method- Principle, Methods, Significance basic culture technique streak plate Grinding
Feb 06, 2019 · The streak plate method is a rapid qualitative isolation method. The techniques commonly used for isolation of discrete colonies initially require that the number of organisms in the inoculums be reduced. It is essentially a dilution technique that involves spreading a loopful of culture over the surface of an agar plate.Streak Plate Method - Explained - LaboratoryInfo basic culture technique streak plate GrindingMay 10, 2021 · The streak plate method requires the number of organisms in the inoculums be reduced. The procedure includes a dilution technique which requires spreading a loopful of culture over the agar plate surface. This is to make sure that the individual cells fall apart on the agar medium surface so as separation of the different species takes place.Estimated Reading Time: 5 minsStreak Plate Method (Procedure) : Microbiology Virtual Lab basic culture technique streak plate GrindingTo streak a specimen from a culture tube, metal transfer loops are first sterilized by flaming the wire loop held in the light blue area of a Bunsen burner just above the tip of inner flame of the flame until it is red-hot. If a hot incinerator is available, the loop may be sterilized
STREAK PLATE CULTURE TECHNIQUE FOR THE ISOLATION
Apr 07, 2018 · In Streak plate technique, a sterilized loop or transfer needle is dipped into the mixed culture of the specimen or a suitably diluted suspension of organisms or Broth cultures, which is then streaked on the surface of Sterile Media plates to make parallel, non-overlapping streaks which are then incubated at optimum temperatures for 24-48 hours usually (for bacteria) or for few weeks in case of Reviews: 2Estimated Reading Time: 10 minsSI Streaking for Isolation - MOspace HomeStarting the streak on the side of the plate farthest from your dominant hand, pass the loop on the surface of the agar in a zig zag pattern filling the surface of the plate.Related searches for basic culture technique streak plate Grstreak plate technique stepsstreak plate technique quizletbacteria streak plate techniqueSome results are removed in response to a notice of local law requirement. For more information, please see here.
Pure culture techniques - SlideShare
May 01, 2016 · Insert the loop into the test tube and remove loop full of broth with bacterial culture using aseptic techniques. Put the loop full of culture on the sterile agar plate. Spread the culture with L- shaped glass on the surface of agar by rotating the Petri-Plate and L-shaped glass both. Keep the lid over the plate as much as possible.Online Course Basic Culture Media and Isolation laboratorians with basic culture media and isolation techniques used in the microbiology laboratory. Laboratorians will review the different types and forms of culture media, correlate culture media with specimen type, describe the steps of streaking a plate, and identify common problems that occur with culture media and isolation techniques basic culture technique streak plate GrindingMicrobiology lab report - StuDocuStreaking is the process of isolating single colonies of an organism on an agar plate 2 , therefore what we expected to observe was isolated, single colonies of our culture in the fourth quadrant. The microorganisms seemed to become more isolated after each quadrant on the agar plate, which can be observed in Figure 2.0, therefore is what was basic culture technique streak plate Grinding
Microbiology Lab 1 Write up.docx - Identifying Different basic culture technique streak plate Grinding
Identifying Different Bacteria Through Basic Slanting and Culturing Techniques s Shape Beaded Filiform Filiform Effuse Consistency Dry Mucoid Dry Buttery Table 4. Bacterial streak plates incubated at 25 ° C for 48 hours of species B, species C, species D, species E with characteristics of size, colour, shape, margin and elevation. D, species E with characteristics of size, colour, shape basic culture technique streak plate GrindingMicrobiological Laboratory Techniques | Clinical GateMar 02, 2015 · To culture a microorganism a small sample, the inoculum, is introduced into a culture medium usually with a platinum wire probe streaked across its surface. This process is called inoculation and the growth that appears on or in the medium is the culture. A culture can be purecontaining one type of organism, or mixedcontaining two or more basic culture technique streak plate GrindingMethods of Culturing Chapter 3 MicroorganismsFive basic techniques 4 Fig. 3.1 A summary of the general laboratory techniques . 1. Inoculate 2. Incubate 3. Isolation 4. Inspection 5. Identification 5 Fig. 3.2 Isolation technique 6 Fig. 3.3 Methods for isolating bacteria.
Making a streak plate - practicalbiology.org
1Two other approaches when making a streak plate are: ito lift the lid vertically (i.e. still directly above the base) the least amount that will allow access of the loop iito place the lid on the working surface, lift out the base, invert it and inoculate the upwards-facing agar surface. This is the method used by professional microbiologists and is best reserved for older students working in a relatively dust and draught-free laboratory. 2You could photSee more on practicalbiology.orgLAB 2: Staining and Streaking - UMDcolonies on this second plate will indicate a pure culture. Most microbiologists repeat streaking from an isolated colony two to three times before they guarantee culture purity. ( Figures 4.21, 4.51, 4.54, 4.55) The use of the streak plate technique requires knowledge of rules of aseptic technique. Whenever working with a culture, the basic culture technique streak plate GrindingImages of Basic culture Technique Streak plate Grinding See allSee all images
Researchers commonly use streak plates to isolate single colonies. A streak plate is actually a serial dilution of an existing culture on solid media. Researchers begin a streak by picking up a small sample of yeast or another microorganism with a sterile instrument, which could be a Chapter 3 Methods of Culturing MicroorganismsFig. 3.2 Isolation technique Three basic methods of isolating bacteria. a) STREAK PLATE Loop Dilution Spread Plate. 2 Media Classified according to three properties Physical state Chemical composition Functional types Culture Media: -Agar a complex polysaccharide from algae is Basic Pure Culture TechniquesThe streak plate method is a rapid and simple technique of mechanically diluting a relatively large concentration of microorganisms to a small, scattered population of cells. The goal is to obtain isolated colonies on a large part of the agar surface, so that desired species can then be brought into pure culture.